In Vitro Embryo Production of Doe Rabbits Administrated with Aqueous Garlic Extract

Document Type : Original research paper

Authors

1 1Department of Animal Production, Faculty of Agriculture, Tanta University, Egypt

2 Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.

3 Department of Animal Production, Faculty of Agriculture, Tanta University, Egypt

Abstract

The aim of the present work was evaluating the effect of daily oral administration of aqueous garlic extract, AGE (5 and 10 mg/kg LBW) for 30-day pre-mating on ovarian activity, and in vitro maturation and fertilization of rabbit does. Rabbit does (n=30) were allocated into three experimental groups (10/group), does in the 1st group (G1) were orally treated with distilled water (2 ml), while, those in the 2nd and 3rd groups were orally treated with 5 mg AGE/kg LBW (G2) or 10 mg AGE/kg LBW (G3) dissolved in 2 ml distilled water. Does were daily administrated before morning feeding for 30 days as a treatment period. Results indicated insignificant effect of AGE on LBW but absolute or relative ovarian weights were higher (P<0.05) by 31.6 and 36.7% in G2 and G3 compared with G1. AGE administration increased (P<0.05) large follicles number, yield, recovery rate, and quality of oocytes, the percentage of embryos at morula stages.
Daily oral administration of 10 mg aqueous garlic extract/kg LBW pre-mating improved ovarian activity, and both in vitro maturation and fertilization of rabbit does. This can be used as a tool for in vitro embryo production in rabbits with different breeds or lines.

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