Effect of RsPod1EGY Phage on Ralstonia solanacearum In Vitro

Document Type : Original research paper

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Abstract

The bacterium Ralstonia solanacearum causes a serious wilt disease in potato plants, and it survives in soil, potato residues, and weeds for a long time. Bacteriophages offer an environmentally beneficial biocontrol method for these bacteria. In this investigation, the RsPod1EGY phage was initially isolated from Egyptian soil and propagated, and its host range was assessed against a wide range of pathogenic and antagonistic bacteria. The results revealed a unique specificity of the RsPod1EGY phage to the R. solanacearum strains used in this study. Furthermore, the RsPod1EGY phage showed in vitro high stability with various activities through a wide range of temperatures (2 up to 60 oC), pH (2 up to 10), and NaCl concentrations (2 up to 10%). In conclusion, the RsPod1EGY phage has an in vitro potential to suppress the growth of R. solanacearum, which may allow this phage to be applied in the field for the biocontrol strategy of this bacteria.

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Journal of Sustainable Agricultural and Environmental Sciences
Volume 3, Issue 3
Articles presented in The first international conference of Faculty of Agriculture Tanta University 6-7 March 2024 entitled: Agriculture and achieving food security in light of SDS 2030.
2024
Pages 75-81

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