Genetical Identification of Salt Tolerant Rhizobium Strain isolated from Faba bean

Document Type : Original research paper

Authors

1 Genetic Department, Faculty of Agricultural, University of Tanta, Tanta, Egypt

2 Biological and Environmental Sciences Department, Faculty of Home Economics, Al-Azhar University, Egypt.

3 Genetics Department, Faculty of Agriculture, Tanta University, 31527 Tanta, Egypt

Abstract

Soil salinity is an environmental concern for global agriculture, it is a limited factor for symbiosis and legume growth and yield. Inoculation with salt-tolerant Rhizobium mitigate significantly the effect of salt on symbiosis and plant growth. Rhizobium is part of complex microbiomes that exist endophytically in root nodules of leguminous plants with high host specification. In this study, the aim is to isolate salt tolerant Rhizobium strain and molecular identification. We isolated Rhizobium strains from Vicia faba that were treated with salt soil extract and irrigated with NaCl (100 mM). The isolate was tested on different concentrations of NaCl (100 and 150 mM NaCl). Rhizobium isolate that is tolerant to salinity identified with molecular technique. The 16S ribosomal RNA gene sequence alignment showed that the strain isolated bacteria is Rhizobium Rosettiformans with a similar rate of 97.27%. Such Rhizobium strains could be useful in stimulating good bean genotype cultivation in moderately saline soils.

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