Determination of Phenolic Compounds in the Leaves Extract of Willow (Salix babylonica L.)

Document Type : Original research paper

Author

Department of Animal Production, Faculty of Agriculture, Tanta University, Egypt

Abstract

Willow (Salix babylonica L.) leaf extract are rich in many bioactive compounds such as phenolic and flavonoid compounds. Thise study aimed to determine the chemical composition and the total phenolics and flavonoids content in willow leaf extract. A chemical analysis of willow leaf was per-formed. Total phenolics and flavonoids in the leaf extract of willow were determined spectrophotomet-rically. Separation and identification of phenolic compounds were determined by High Performance Liquid Chromatography (HPLC). The content of crude protein, ether extract, crude fibers, ash, and available carbohydrate were 11.6, 3.4, 23.5, 14.1, and 38.6 %, respectively. Total phenolic and flavo-noid contents in leaf extract were 75.30 mg GAE/g and 29.6 mg CE/g dried extract, respectively. Gal-lic acid, chlorogenic acid, caffeic acid, syringic, coumaric acid, hydroxybenzoic, cinnamic acid, cate-chin, rutin, naringenin, and quercetin were detected in leaf extract, while the major bioactive com-pounds in the leaf extract were chlorogenic acid (2.51mg/g) and rutin (1.94 mg/g). It could be con-cluded that willow leaf extract was a rich source of natural phenolics and flavonoid compounds. Therefore, willow leaf extract can be used as an antioxidant agent in pharmaceutical products, antimi-crobial, anti-inflammatory, food preservatives, and animal diets.

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