Molecular Identification Of Lead-Resistant Bacteria And Assessment Of Their Effects On Vicia Faba Planted In Lead-Contaminated Soil.

Document Type : Original research paper

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1 Biological and Environmental Sciences Department Faculty of Home Economics Al-Azhar Unv.

2 Biological and Environmental Sciences Department, Faculty of Home Economics, Al-Azhar University, Egypt.

Abstract

Bioremediation is a crucial tool for managing soil contamination. In this study, we aimed to reduce the side effects of lead (Pb) on Vicia faba seedling using Pb tolerant bacteria strains isolated from pol-luted places and to study these effects with Pb. Four Pb-tolerant isolates were selected from twenty-two strains. The isolated bacteria were identified using 16S rRNA gene sequence technique. Identified strains were classified as members of the genus Bacilli. The tolerant isolates were Brevibacillus parabrevis, Brevibacillus reuszeri, Bacillus subtilis and Bacillus amyloliquefaciens. The results indicated to the side effects of Pb on shoot fresh and dry weight, root fresh and dry weight, shoot and root length, proline content, catalase, peroxidase and polyphenol oxidase activities as well as cytogenetical measurements. Lead inhibited the mitotic index and caused an increase in chromosomal aberrations. The treatment with four bacteria significantly reduced the side effects of Pb on the level of growth and physiological pa-rameters. Also, it improved the mitotic index and reduced the chromosomal aberrations.

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