Effect of Addition of Pomegranate Juice (Punica Granatum) in Tris-based Extenderon Physical, Kinetic Parameters of Spermatozoa in Cryopreserved Ossimi Ram Semen
1
Animal Production Department, Faculty of Agriculture, Tanta University, Egypt
2
Animal Production Research Institute, Agricultural Research Center, Egypt
Abstract
Pomegranate juice (PJ) contains potent antioxidant activity against lipid peroxidation. The current study's objective was to ascertain the effects of various pomegranate juice concentrations (2.5, 5, 7.5 and 10%) in a tris-based extender on physical and kinetic spermatozoa post-dilution, equilibration time, and freezing of Ossimi ram semen. Semen samples were taken once each week for seven weeks from five rams. Semen was diluted at a rate of 1:20, equilibrated for 4 hours at 5 °C, and then was loaded into 0.25 mL straws before being frozen in liquid nitrogen. By using spectroscopic assays, it was possible to measure the physical, kinetic, enzyme, and antioxidant capacities of spermatozoa. After thawing, the 10% pomegranate juice addition to the extender significantly (p 0.05) improved the morphology of the semen (CASA, total motility, progressive motility, VAP, VSL, VCL, STR and LIN). It is concluded that the addition of 10% pomegranate juice to tris-based extender enhanced post-thawing semen characteristics and CASA motion dynamics of cryopreserved Ossimi ram semen while also increasing overall antioxidant capacity and reducing MDA concentration.
Gabr, A. A., Hammad, M. E., El-Sherbieny, M. A., Ouda, A. B. A., & Yousif, A. I. A. (2022). Effect of Addition of Pomegranate Juice (Punica Granatum) in Tris-based Extenderon Physical, Kinetic Parameters of Spermatozoa in Cryopreserved Ossimi Ram Semen. Journal of Sustainable Agricultural and Environmental Sciences, 1(1), 65-79.
MLA
A. A Gabr; M. E Hammad; M. A El-Sherbieny; A. B. A Ouda; A. I. A Yousif. "Effect of Addition of Pomegranate Juice (Punica Granatum) in Tris-based Extenderon Physical, Kinetic Parameters of Spermatozoa in Cryopreserved Ossimi Ram Semen", Journal of Sustainable Agricultural and Environmental Sciences, 1, 1, 2022, 65-79.
HARVARD
Gabr, A. A., Hammad, M. E., El-Sherbieny, M. A., Ouda, A. B. A., Yousif, A. I. A. (2022). 'Effect of Addition of Pomegranate Juice (Punica Granatum) in Tris-based Extenderon Physical, Kinetic Parameters of Spermatozoa in Cryopreserved Ossimi Ram Semen', Journal of Sustainable Agricultural and Environmental Sciences, 1(1), pp. 65-79.
VANCOUVER
Gabr, A. A., Hammad, M. E., El-Sherbieny, M. A., Ouda, A. B. A., Yousif, A. I. A. Effect of Addition of Pomegranate Juice (Punica Granatum) in Tris-based Extenderon Physical, Kinetic Parameters of Spermatozoa in Cryopreserved Ossimi Ram Semen. Journal of Sustainable Agricultural and Environmental Sciences, 2022; 1(1): 65-79.
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